| 质粒名称: | pcdna3 flag - ULK1 wt |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 27636 |
| 存储实验室: | Reuben Shaw |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5500 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mouse ULK1 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 3150 bp |
| 基因突变: | Initial M from ULK1 removed. |
| GenBank编号 | NP_033495.2 |
| 启动子 | -- |
| 标签/融合蛋白: | flag（主干上的N终端） |
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | attR1 (销毁后未知) |
| 3' 克隆位点: | attR2(销毁后未知) |
| 5' 测序引物 | t7 promotor |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pcdna3 flag - ULK1 wt was a gift from Reuben Shaw (Addgene plasmid # 27636 ; http://n2t.net/addgene:27636 ; RRID:Addgene_27636) |
| 参考文献部分: | Phosphorylation of ULK1 (hATG1) by AMP-Activated Protein Kinase Connects Energy Sensing to Mitophagy. Egan DF, Shackelford DB, Mihaylova MM, Gelino SR, Kohnz RA, Mair W, Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J, Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ. Science. 2010 Dec 23. ():. 10.1126/science.1196371 PubMed 21205641 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcdna3 flag - ULK1 wt 8636 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pcdna3 flag - ULK1 wt
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8636)
AUTHORS Egan DF, Shackelford DB, Mihaylova MM, Gelino SR, Kohnz RA, Mair W,
Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J,
Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ
TITLE Phosphorylation of ULK1 (hATG1) by AMP-Activated Protein Kinase
Connects Energy Sensing to Mitophagy.
JOURNAL Science. 2010 Dec 23. ():.
PUBMED 21205641
REFERENCE 2 (bases 1 to 8636)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8636
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 629..648
/label=T7
/note="T7 promoter, forward primer"
promoter 629..647
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 660..669
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 669..692
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
protein_bind 705..729
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(3887..3911)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
promoter complement(3955..3973)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(3956..3973)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(3993..4010)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3999..4223
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4269..4697
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4356..4375)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4566..4587
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4706..4726)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4711..5040
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4891..5026
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4953..4972
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 5107..5901
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(5161..5180)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5771..5790
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 6075..6196
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6112..6131)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6166..6185
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(6245..6261)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6245..6261)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6258..6280)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6269..6285
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6293..6323)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6338..6359
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6476..6493)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6647..7235)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6727..6746)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7406..8266)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8029..8048
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8267..8371)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8446..8465)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
661 ccaccatgga ctacaaggac gacgacgata aggaattcgt tatcacaagt ttgtacaaaa
721 aagcaggctt cgagccgggc cgaggcggcg tcgagaccgt gggcaagttc gagttctctc
781 gcaaggacct gattggacac ggcgccttcg cggtggtctt caagggtcga caccgcgaga
841 agcacgacct ggaggtggcc gtcaaatgca ttaacaagaa gaaccttgcc aagtcccaaa
901 cactgctggg aaaggaaatc aaaatcctga aggaactaaa gcacgaaaac atcgtggcgc
961 tgtatgactt ccaggaaatg gctaattctg tctacctggt catggagtat tgtaatggtg
1021 gagacctggc tgactacctg cacactatgc gcacactgag tgaagacact gtcaggcttt
1081 tcctacagca gatcgctggc gccatgcggc tgctgcacag caagggcatc atccaccggg
1141 acctgaagcc ccaaaacatc ctgctgtcca accctggggg ccgccgggcc aaccccagca
1201 acatccgagt caagattgct gactttggat tcgctcggta cctccagagc aacatgatgg
1261 cggccacact ctgtggttct cctatgtaca tggctcctga ggtcattatg tcccagcact
1321 acgatggaaa ggctgacctg tggagcattg gcaccattgt ctaccagtgt ctgacaggga
1381 aggccccttt tcaggccagc agccctcagg atttgcgcct gttttatgag aagaacaaga
1441 cactagttcc tgccatcccc cgggagacat cagctcccct gcggcagctg ctcctggctc
1501 tgttgcagcg gaaccacaag gaccgcatgg actttgatga atttttccac caccctttct
1561 tggatgccag cacccccatc aagaaatccc cacctgtgcc tgtgccctca tatccaagct
1621 cagggtctgg cagcagctcc agcagcagct ctgcctccca cctggcctct ccaccgtccc
1681 tgggggagat gccacagcta cagaagaccc ttacctcccc agccgatgct gctggctttc
1741 ttcagggctc ccgggactct ggtggcagca gcaaagactc ctgtgacaca gatgactttg
1801 tcatggtccc agcccagttt ccaggtgatc tagttgctga ggcagccagt gccaagcccc
1861 cacctgatag cctgctgtgt agtgggagct cattggtggc ctctgctggc ctagagagcc
1921 acggccgtac cccctctccc tctccgacct gcagcagctc tcccagcccc tctggccggc
1981 ctggcccctt ctccagcaac aggtacggtg cctcggtccc cattcctgtc cccactcagg
2041 tgcacaatta ccagcgcatc gagcaaaacc tgcaatcgcc cactcaacag cagacagcca
2101 ggtcctctgc catccgaagg tcagggagca ccagccccct gggctttggc cgggccagcc
2161 catcaccccc ctcccacacc gatggagcca tgctggccag gaagctgtca cttggaggtg
2221 gccgtcccta cacaccttct ccccaagtgg gaaccatccc agagcgaccc agctggagca
2281 gagtgccctc cccacaagga gctgatgtgc gggttggcag gtcaccacga cccggttcct
2341 ctgtgcctga gcactctcca agaaccactg ggctgggctg ccgcctgcac agtgccccta
2401 acctgtccga cttccatgtt gtgcgtccca agctgcctaa gcccccaaca gacccactgg
2461 gagccacctt tagcccaccc cagaccagcg caccccagcc atgcccaggg ctacagtctt
2521 gccggccact gcgtggctca cctaagctgc ctgacttcct acagcggagt cccctacccc
2581 ccatcctagg ctctcctacc aaggccgggc cctcctttga cttccccaaa acccccagct
2641 ctcagaattt gctgaccctg ttggctaggc agggggtagt aatgacacca cctcggaacc
2701 gtacactgcc tgacctctcc gaggccagtc ctttccatgg ccagcagctg ggctctggcc
2761 ttcggcccgc tgaagacacc cggggtccct ttggacggtc cttcagcacc agccgcatta
2821 cggacctgct gcttaaggct gcatttggga ctcaggcctc tgactcaggc agcacagaca
2881 gcctacagga gaaacctatg gagattgctc cctctgctgg ctttggaggg actctgcatc
2941 caggagctcg tggtggaggg gccagcagcc cagcacctgt ggtatttact gtaggctccc
3001 cacccagtgg tgccacccca ccccagagta cccgtaccag aatgttctca gtgggctctt
3061 ccagctccct gggctctact ggctcctcct ctgcccgcca cttagtgcct ggggcctgtg
3121 gagaggcccc ggagctttct gccccaggcc actgctgtag ccttgctgac ccccttgctg
3181 ccaacttgga gggggctgtg accttcgagg ctcctgacct cccagaggag accctcatgg
3241 agcaagagca cacggaaacc ctacacagtc tgcgcttcac actagcgttt gcacagcaag
3301 ttctggagat tgcagccctg aagggaagtg ccagtgaggc cgccggtggc cctgagtacc
3361 agctccagga aagtgtggtg gctgaccaga tcagtcagtt gagccgagag tggggctttg
3421 cagagcaact ggttctgtac ttgaaggtgg ctgagctgct gtcctcaggc ctacagactg
3481 ccattgacca gattcgagct ggcaaactct gcctttcatc tactgtgaag caggtggtac
3541 gcagactaaa tgagctgtac aaggccagcg tggtatcctg ccagggcctc agcttgcgac
3601 ttcagcgctt ctttctggac aaacaacggc tgctggacgg gatccatggt gtcactgcag
3661 agcggctcat cctcagccat gctgtgcaaa tggtacaatc agctgccctt gatgagatgt
3721 tccagcaccg agagggctgt gtaccgagat atcacaaagc cctgctattg ctggaggggt
3781 tgcagcacac tctcacggac caggcagaca ttgagaacat tgccaaatgc aagctgtgca
3841 ttgagaggag actctcggcc ctgctgagtg gtgtctatgc ctgaataccc agctttcttg
3901 tacaaagtgg tgataacgtg gatcccactc gagcatgcat ctagagggcc ctattctata
3961 gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
4021 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
4081 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
4141 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
4201 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
4261 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
4321 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
4381 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
4441 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
4501 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
4561 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
4621 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
4681 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
4741 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
4801 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
4861 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
4921 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
4981 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
5041 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
5101 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
5161 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
5221 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
5281 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
5341 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
5401 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
5461 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
5521 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
5581 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
5641 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
5701 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
5761 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
5821 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
5881 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
5941 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
6001 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
6061 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
6121 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
6181 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
6241 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
6301 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
6361 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
6421 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
6481 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
6541 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
6601 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
6661 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
6721 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
6781 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
6841 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
6901 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
6961 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
7021 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
7081 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
7141 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
7201 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
7261 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
7321 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
7381 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
7441 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
7501 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
7561 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
7621 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
7681 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
7741 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
7801 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
7861 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
7921 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
7981 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
8041 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
8101 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
8161 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
8221 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
8281 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
8341 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
8401 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
8461 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
8521 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
8581 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgtt
//
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